An investigation of slaughter weight and muscle type effects on carcass fatty acid profiles and meat textural characteristics of young Holstein Friesian bulls.

Study objectives included the assessment of carcass fatty acid composition and meat texture characteristics of younger Holstein Friesian bulls. Three experimental groups were formed based on the weights of the 23 young bulls at slaughter: lighter, medium, and heavier. Samples were taken from the Gluteus medius (GM) and Longissimus thoracis muscles 24 h after slaughter. Fatty acid composition, Warner-Bratzler Meat Shear (WBS) measurements, as well as textural profile analysis (TPA) and sensory analysis of the muscle samples were conducted. The fatty acid composition was determined using Thin Layer Chromatography (HPTLC). Polyunsaturated fatty acids and dietary fatty acids give a neutral hypocholesterolemic effect in direct fluorescent antibody (DFA) contents, DFA/OFA (C14:0+C16:0) ratio, hardness, Warner-Bratzler Shear force and also the chews number - which is desirable - before swallowing (NCBS) the meat were significantly decreased with the increasing slaughter weight. Higher slaughter weight resulted in a larger amount of beef with a better panel tenderness score; however, the meat obtained from the LSW group was less healthy considering the fatty acid profile. Additionally, internal fat contained the highest saturated fatty acids concentrations, while subcutaneous fat contained the highest amount of monounsaturated fatty acids. Furthermore, intramuscular fat levels were highest in PUFA and PUFA/SFA ratio. As a result, this study strongly suggests that slaughter weight and anatomical location of fat samples contribute significantly to meat texture characteristics and fatty acid profiles in Holstein Friesian bulls.

Effects of flooring types on teat end bacteria counts, milk quality, hygiene and behaviour of dairy cows housed in tie-stall closed barn.

This study aimed to investigate environmental mastitis causing bacteria counts in the teat end, somatic cell counts (SCC) of milk samples, cleanliness scores and behavior of cows kept on concrete and rubber mat floorings. For this purpose, 19 Holstein-Friesian dairy cows were allocated into concrete and rubber mat groups. Swab samples were taken from the teat ends to determine the bacterial counts causing environmental mastitis. Milk samples were collected from a composite of all four quarters to determine the SCC. Instantaneous sampling method was utilized to observe the behavioral activities of cows. Cows were visually evaluated to determine the udder cleanliness score. Independent samples t-test was utilized in the statistical analysis of the obtained data. Coliform (P<0.05), Escherichia coli, and Klebsiella spp. (P<0.01) counts of the swab samples taken from the cows housed on concrete flooring were significantly higher than rubber mat group. However, no statistically significant differences were found between groups in terms of total bacteria, Streptococcus spp., and Enterobacteriaceae counts. The SCC on samples taken from cows kept on concrete surface were significantly higher (P<0,05) than that of animals housed on rubber mat. Furthermore, cows in the rubber mat group were determined to be significantly cleaner (P<0.05) than those in concrete group. It was also determined that the cows housed on rubber mat spent significantly longer time for lying behavior (P<0.05), which is a significant indicator of animal comfort. The time spent for standing without eating was considerably higher (P<0.01) in concrete group. In addition, the times spent for eating was significantly lower (P<0.01) in the concrete group. It was concluded that, using rubber mat instead of concrete for flooring in tie-stall barns decreases the contamination of environmental mastitis pathogens, increases milk quality and cow cleanliness score as well as animal comfort and welfare.

Effects of Slaughter Age of Holstein Friesian Bulls on Meat Quality: Chemical Composition, Textural Characteristics, Sensory Attributes and Fatty Acid Profile.

This study aimed to investigate the effects of slaughter age (young vs. old), muscle type (Longissimus dorsi (LD), Gluteus medius (GM)) and fat deposits (kidney knob and channel fat, subcutaneous fat, intramuscular fat) on chemical, organoleptic, textural characteristics and fatty acid composition of Holstein Friesian bull meat. For this purpose, the carcasses of 26 Holstein Friesian bulls that had been fattened on the same private farm were assigned to two experimental groups based on their age at slaughter: a young group (YG) (average age: 17.0 ± 1.0 months old) and an old group (OG) (average age: 22.0 ± 1.0 months old). The percentage of crude protein, panel tenderness score, polyunsaturated fatty acid (PUFA) and saturated fatty acid (SFA) content, the PUFA/SFA ratio and the hypocholesterolemic fatty acid (DFA)/hypercholesterolemic fatty acid (OFA) ratio of the bull carcasses decreased significantly with increasing slaughter age. By contrast, the OFA content of the carcasses significantly increased (p < 0.05) with increasing slaughter age. Advanced slaughter age resulted in lower panel tenderness scores. Additionally, the meat of the bulls in the OG was considered to be less healthy because of the less desirable fatty acid composition and nutritional indices, such as the PUFA/SFA and hypocholesterolemic/hypercholesterolemic ratios, compared to the meat from the bulls in the YG. Furthermore, the intramuscular fat and internal fat contained high percentages of PUFA and SFA and high PUFA/SFA and hypocholesterolemic/hypercholesterolemic ratios. Interestingly, the percentage of OFA content in the internal and intramuscular fat tissues decreased with increasing slaughter age. In conclusion, this study provided evidence that slaughter age and muscle and fat type are essential sources of variations in the textural characteristics, sensory panel attributes and fatty acid profile of meat from Holstein Friesian bulls.

Effects of slaughter age and muscle type on meat quality characteristics of Eastern Anatolian Red bulls.

The effects of slaughter age and muscle type on meat quality properties of Eastern Anatolian Red (EAR) bulls ( n = 46 ) were investigated in the present study. Forty-six EAR bulls were slaughtered at 15, 17, 19, 25 and 27 months. Meat samples were taken from longissimus dorsi (LD) and gluteus medius (GM) muscles obtained from the carcasses at 24 h post-mortem. Meat color parameters were significantly affected by slaughter age. Older animals (19, 25 and 27 months of age) possessed higher  L * , a *  and H  values than those of younger animals (15 and 17 months of age). LD muscle had higher  L * and  a * values than those of GM muscles. The influences of slaughter age and muscle type on the proportions of dry matter, ether extract, crude ash and crude protein were not significant in the present study. The influence of slaughter age on the tenderness, acceptability, juiciness, number of chews and Warner-Bratzler shear (WBS) values were found to be significant. Tenderness, juiciness, flavor intensity and acceptability increased until 19 months of age, and then increase in age resulted in lower tenderness, flavor intensity and acceptability scores. Cooking yield significantly increased depending on the increase of age. WBS and cooking yield values from the LD were higher than that of the GM muscle. Textural characteristics such as hardness, springiness, cohesiveness, gumminess, chewiness and resilience were not affected by slaughter age, but muscles had a significant influence on hardness, gumminess, chewiness and resilience. In conclusion, most eating quality characteristics and color parameters were positively influenced by slaughter age.

Effects of different finishing systems on carcass traits, fatty acid composition, and beef quality characteristics of young Eastern Anatolian Red bulls.

The effects of three finishing systems on carcass composition and other characteristics of meat were studied in 15 young Eastern Anatolian Red bulls in Eastern Turkey. For 93 days, five bulls grazed pasture (P), five grazed the same pasture and were given concentrate supplementation (P + C), and five grazed the same pasture for 93 days, and then for an additional 40 days, they received concentrate in a barn (PF). Carcasses of the P bulls were lighter and leaner than those of the PF animals. Compared with the P carcasses, PF carcasses had a greater percentage of kidney, pelvic, and heart fat (P < 0.05), marbling score (P < 0.05), fat thickness over the longissimus dorsi (LD) muscle (P < 0.05), and percentage of ether extractable lipids (P < 0.05). The LD of P carcasses was darker (P < 0.01) in color and the meat was tougher. The ratio of polyunsaturated to saturated fatty acids in LD intramuscular fat was higher (P < 0.05) for P bulls than for P + C and PF. P carcasses contained a lower (P < 0.05) proportion of saturated fatty acids than PF carcasses. It is concluded that pasture-based finishing results in a more favorable fatty acid profile of meat and produces a healthier food, but the meat is darker and tougher.

Fattening performance, carcass traits and meat quality characteristics of calves sired by Charolais, Simmental and Eastern Anatolian Red sires mated to Eastern Anatolian Red dams.

Comparisons were made among calves sired by Charolais (C), Simmental (S) and Eastern Anatolian Red (EAR) breeds of bulls for fattening, carcass and meat quality traits when mated to EAR dams. C- and S-sired calves had 43.1% and 36.4% higher daily weight gain, 44.5% and 43.9% heavier final weight in fattening, respectively. Calves produced by C sires had best feed efficiency value (6.51 vs. 7.44 and 7.22) compared to the S and EAR sire breed groups. Carcasses of C- and S-sired calves had heavier weight, higher dressing percentage and greater Longissimus dorsi (LD) muscle area than those of EAR-sired calves. USDA yield grades were lower (P<0.01) for carcasses from C and S sires, and highest for carcasses from EAR calves. C-sired calves received higher (P<0.01) ratings for panel tenderness score, lower shear force value and number of chews before swallow than S- and EAR-sired progeny. Overall results of the study suggested that fattening performance, carcass and meat quality characteristics might be considerably improved by using C sires in the crossbreeding program as sire breed.